I have installed a new XenApp 6.5 Farm with Local Profiles and AppSense User Virtualization. So I had the problem that on the first login the mouse movement in a Published Application was very, very sluggish. So after two or three days full of testing I have found that the following key seems to be a problem: HKCU\Control Panel\Desktop\UserPreferencesMask
After some more searching in the Internet I have found the following forum entry which gave me the sollution: http://forums.citrix.com/thread.jspa?threadID=261090
As a short compendium you have to make a AppSense Logon Configuration or a Group Policy Preference which sets the above registry key to hex : 90,12,01,80. I have provided a screenshot how it looks like:
This should solve your problem with the sluggish mouse movements.